BIO-13

Synergy H1m Plate Reader

NanoMaterials Facility

MAKE

BioTek

MODEL

Synergy H1m Monochromator-Based Multi-Mode Microplate Reader

LOCATION

G05 LISE

This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection in a variety of applications, such as: cell viability, growth, toxicity, ELISA, caspase and DNA assays. To create the ideal physiological environment for live-cell assays, this plate reader has a user-adjustable orbital shaking and advanced 4-ZoneTM temperature control (from ambient to 45°C +0.5°C). Optional add-ons:

1. a new Gas Controller for Synergy H1m allows control and monitoring of CO2 and O2 levels within the Synergy H1

2. a dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).

Detection mode: Fluorescence, Luminescence, UV-Visible

Absorbance, TRF (secondary mode) Absorbance -microplate types: 1- to 384-well plates Compatible with Take3 plate with 2 µL microspots -wavelength range: 230 – 999 nm, 1 nm increment -dynamic range: 0 – 4.0 OD -resolution: 0.0001 OD

Fluorescence -fluorescence sensitivity: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) -wavelength selection: Double grating monochromators (Top and Bottom) -wavelength range: 250 – 700 nm (850 nm option) -dynamic range: 5 decades

Luminescence -sensitivity: 20 amol ATP typical (flash) 100 amol ATP (glow) typical -wavelength range 300 – 700 nm -dynamic range >6 decades

Contact staff for training information.

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